Selected article for: "forward primer and real time"
Author: Congliang Miao; Jinqiang Zhuang; Mengdi Jin; Huanwen Xiong; Peng Huang; Qi Zhao; Li Miao; Jiang Du; Xinying Yang; Peijie Huang; Jiang Hong
Title: A comparative multi-centre study on the clinical and imaging features of comfirmed and uncomfirmed patients with COVID-19
  • Document date: 2020_3_24
    • ID: j8odxs1h_7
    Snippet: Nasopharyngeal swabs or sputum specimens were collected and sent to the local center for disease control and prevention (CDC) for detection of SARS-Cov-2 by RT-PCR tests. Patients with first negative results should receive repeated tests in an interval of at least 1 day. We used forward primer, reverse primer and probe target to envelope gene of SARS-Cov-2. The sequence of forward primer was 5ʹ-TCAGAATGCCAATCTCCCCAAC-3ʹ; The sequence of reverse.....
    Document: Nasopharyngeal swabs or sputum specimens were collected and sent to the local center for disease control and prevention (CDC) for detection of SARS-Cov-2 by RT-PCR tests. Patients with first negative results should receive repeated tests in an interval of at least 1 day. We used forward primer, reverse primer and probe target to envelope gene of SARS-Cov-2. The sequence of forward primer was 5ʹ-TCAGAATGCCAATCTCCCCAAC-3ʹ; The sequence of reverse primer was 5ʹ-AAAGGTCCACCCGATACATTGA-3ʹ; and The sequence of the probe was 5ʹCY5-CTAGTTACACTAGCCATCCTTACTGC-3ʹBHQ1. Conditions for the amplifications of the real-time RT-PCR were 50°C for 15 min, 95°C for 3 min, followed by 45 cycles of 95°C for 15 s and 60°C for 30 s.

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