In-depth Bioinformatic Analyses of Human SARS-CoV-2, SARS-CoV, MERS-CoV, and Other Nidovirales Suggest Important Roles of Noncanonical Nucleic Acid Structures in Their Lifecycles

Martin Bartas; Václav Brázda; Natália Bohálová; Alessio Cantara; Adriana Volná; Tereza Stachurová; Katerina Malachová; Eva B. Jagelská; Otília Porubiaková; Jirí Cerven; Petr Pecinka

The highest PQS frequencies were found in Beihai Nido-like virus 1, which is an intracellular parasite of sea snails in genus Turritella. Beihai Nido-like virus 1 has in total 184 PQSs in its genomic sequence 20,278 nt long and PQS frequency of 9.07 PQS per 1,000 nt. On the other hand, no PQSs were found in the genomic sequences of 16 other Nidovirales species. The mean PQS value for all Nidovirales was This is a provisional file, not the final typeset article 0.48 PQS per 1,000 nt. By viral families, the highest mean PQS frequency per 1,000 nt was in Arteriviridae (1.18), followed by in Mesnidovirineae (1.03), much lower in Tobaniviridae (0.43) and