Selected article for: "Assay kit and Protein Assay kit"

Author: Luis M. Colon-Perez; Kristen R. Ibanez; Mallory Suarez; Kristin Torroella; Kelly Acuna; Edward Ofori; Yona Levites; David E. Vaillancourt; Todd E. Golde; Paramita Chakrabarty; Marcelo Febo
Title: Increased Neurite Orientation-Dispersion and Density in the TgCRND8 Mouse Model of Amyloidosis: Inverse Relation with Functional Connectome Clustering and Modulation by Interleukin-6
  • Document date: 2019_2_27
  • ID: hyeygk64_41
    Snippet: Immunohistochemically stained sections were captured using the Scanscope XT image scanner (Aperio) and analyzed using the ImageScope program. Brightness and contrast alterations were applied identically on captured images using Adobe Photoshop CS3. Snap-frozen forebrains (without olfactory bulb and cerebellum) were homogenized in RIPA Buffer (Boston Bioproducts) and the homogenate was centrifuged at 100,000 x g for 1 h at 4°C. Protein concentrat.....
    Document: Immunohistochemically stained sections were captured using the Scanscope XT image scanner (Aperio) and analyzed using the ImageScope program. Brightness and contrast alterations were applied identically on captured images using Adobe Photoshop CS3. Snap-frozen forebrains (without olfactory bulb and cerebellum) were homogenized in RIPA Buffer (Boston Bioproducts) and the homogenate was centrifuged at 100,000 x g for 1 h at 4°C. Protein concentration in the supernatant was determined using BCA Protein Assay kit (Pierce). mIL-6 levels were evaluated using sandwich capture ELISA assays using the RIPA soluble mouse forebrain lysates as per manufacturer's instructions (BD OptiEIA, BD Biosciences).

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