Author: V’kovski, Philip; Steiner, Silvio; Thiel, Volker
Title: Proximity Labeling for the Identification of Coronavirus–Host Protein Interactions Cord-id: deql0cgj Document date: 2020_5_11
ID: deql0cgj
Snippet: Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein–protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., p
Document: Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein–protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirA(R118G) (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies.
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