Selected article for: "mix enzyme and primer reverse"
Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously
  • Document date: 2020_2_22
    • ID: 0vjs2w3l_11
    Snippet: Quantitative Real-Time PCR (qRT-PCR) was performed to detect the PEDV RNA and λ phage DNA using HiScript II One Step qRT-PCR Probe Kit (Q222-CN, Vazyme) according to the manufacturer's protocol. The reaction (25 µL) was assembled as follows: 12.5 µL of 2 × One Step U+ Mix, 1.25 µL of One Step U+ Enzyme Mix, 0.5 µL of 50 × ROX Reference Dye 1, 0.5 µL of Gene Specific Primer Forward (10 µM), 0.5 µL of Gene Specific Primer Reverse (10 µM).....
    Document: Quantitative Real-Time PCR (qRT-PCR) was performed to detect the PEDV RNA and λ phage DNA using HiScript II One Step qRT-PCR Probe Kit (Q222-CN, Vazyme) according to the manufacturer's protocol. The reaction (25 µL) was assembled as follows: 12.5 µL of 2 × One Step U+ Mix, 1.25 µL of One Step U+ Enzyme Mix, 0.5 µL of 50 × ROX Reference Dye 1, 0.5 µL of Gene Specific Primer Forward (10 µM), 0.5 µL of Gene Specific Primer Reverse (10 µM), 0.25 µL of TaqMan Probe (10 µM), and 4.5 µL nuclease free H2O.

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