Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously

Qingxin Zhang; Qingshun Zhao

In conclusion, our results show that virus inactivation with temperature higher than 56 o C result in the degradation of viral nucleic acids seriously, which will lead to the artificial shortage of detectable templates of viral nucleic acids in samples, and finally lead to false negative of clinical detection in some samples. Preserved with the optimized solution such as R503, the detectable templates of viral nucleic acids can be kept unchanged after the samples were incubated at 56 o C or higher for killing the viruses although the integrity of cellular nucleic acids were obviously disrupted. The results suggest that if the samples were prepared with an optimal solution, the detectable numbers of viral templates would be unchanged because virus inactivation progress with high temperature does not erase all the RNA but leave small fragments of nucleic acids that are good enough for using as the template for qRT-PCR detection.