Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously

Qingxin Zhang; Qingshun Zhao

protecting the detectable amount of coronavirus RNA from disrupting, we first compared the difference of the detectable amount of coronavirus RNA between the two samples that are prepared with R503 and Hank's solution and stored at 4 o C. The results showed that the detectable amount of the viral RNA in the two prepared solutions was similar (Fig. 1G, Table S1 ), and the difference between the mean values of their CT was less than 0.5 (31.64 ± 0.10 vs 31.95 ± 0.10). The results suggest that there is no significant difference in the amount of detectable viral RNA templates between the samples prepared by either R503 or Hank's solutions that were stored at Table S1 ). The results suggest that the detectable templates of viruses in the samples prepared by Hank's solution was only 32.30% of R503 solution after the samples were inactivated at 56 o C for 30 minutes to kill the virus. Furthermore, after inactivation at 92 o C for 5 minutes, the CT of detected PEDV virus in the two preservation solutions were 31.43 ± 0.08 (R503) and 36.84 ± 0.37 (Hank's), respectively. The mean value of CT of detected virus in the samples prepared by Hank's solution was 5.4125 higher than that prepared by R503 (P < 0.0001; Figure 1I , Table S1 ). The results reveal the detectable amount of virus in the samples prepared by Hank's solution was only 2.35% of R503 solution after the samples were inactivated at 92 o C for 5 minutes to kill the virus.