Selected article for: "AGC target and equilibration 10 min"
Author: Charles J Sande; Jacqueline M Waeni; James M Njunge; Martin N Mutunga; Elijah Gicheru; Nelson K Kibinge; Agnes Gwela
Title: In-silico immune cell deconvolution of the airway proteomes of infants with pneumonia reveals a link between reduced airway eosinophils and an increased risk of mortality
  • Document date: 2019_11_13
    • ID: h1zkka8p_19
    Snippet: Each LC run was finished by washout with 98% B for 10 min and re-equilibration in 2% B for 30 min. Five blanks of 40 min each were run on the column between each injection comprising of two wash cycles with 90% B and an equilibration phase of 15 min to avoid sample carryover. Peptides were measured using a Q Exactive Orbitrap mass spectrometer (Thermo Scientific, USA) coupled to the chromatography system via a nano-electrospray ion source (Thermo.....
    Document: Each LC run was finished by washout with 98% B for 10 min and re-equilibration in 2% B for 30 min. Five blanks of 40 min each were run on the column between each injection comprising of two wash cycles with 90% B and an equilibration phase of 15 min to avoid sample carryover. Peptides were measured using a Q Exactive Orbitrap mass spectrometer (Thermo Scientific, USA) coupled to the chromatography system via a nano-electrospray ion source (Thermo Scientific). On the Q Exactive , the ms^1 settings for peptides were: Resolution, 70000; AGC target, 3e6; maximum IT, 120 ms; scan range, 400-1800 m/z; while the ms^2 settings for fragmentation spectra of peptides were:

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