Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously

Qingxin Zhang; Qingshun Zhao

However, 2019-nCoV is a single stranded RNA virus. Theoretically, the progress of killing the viruses with high temperature will damage the integrity of their RNA genomes in the samples, thus improperly reducing the amount of target viral templates, and eventually leading to high false negative rate of the virus detection. In this study, we employed porcine epidemic diarrhea virus (PEDV), a kind of coronavirus, as a model virus to test the above hypothesis and our results demonstrated that the virus inactivation progress by high temperature damaged the detectable template of the coronavirus seriously. Biological. Company, Ltd.) or 1 ng λDNA (3010, Takara, Japan) were suspended and mixed together using 1.5 mL of sample preservation medium (R503, Vamzye, China) or 1.5 mL Hank's solution.