Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously Document date: 2020_2_22
ID: 0vjs2w3l_35
Snippet: Recently, Chen reported that the inactivation by incubating the sample with 56 o C for 30 min to kill the virus had no significant effect on the detection of 2019-nCoV by qRT-PCR [5] . However, combining the limitations such as only two samples tested and higher amount of virus in the two samples as the authors discussed in their researches, and our experimental evidences that the virus inactivation by high temperature did reduce the detectable a.....
Document: Recently, Chen reported that the inactivation by incubating the sample with 56 o C for 30 min to kill the virus had no significant effect on the detection of 2019-nCoV by qRT-PCR [5] . However, combining the limitations such as only two samples tested and higher amount of virus in the two samples as the authors discussed in their researches, and our experimental evidences that the virus inactivation by high temperature did reduce the detectable amount of the porcine coronavirus template seriously, it is highly recommended to carry out systematic investigation on the impact of high temperature inactivation on the integrity of 2019-nCoV nucleic acids and develop a sample preservation solution to protect the detectable templates of 2019-nCoV nucleic acids from high temperature inactivation damage. Table S1 . Figure S1 author/funder. All rights reserved. No reuse allowed without permission.
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