Author: Esmail, Sally; Knauer, Michael J.; Abdoh, Husam; Voss, Courtney; Chin-Yee, Benjamin; Stogios, Peter; Seitova, Almagul; Hutchinson, Ashley; Yusifov, Farhad; Skarina, Tatiana; Evdokimova, Elena; Ackloo, Suzanne; Lowes, Lori; Hedley, Benjamin D.; Bhayana, Vipin; Chin-Yee, Ian; Li, Shawn S.-C.
Title: Rapid and accurate agglutination-based testing for SARS-CoV-2 antibodies Cord-id: bw6o2zl1 Document date: 2021_5_12
ID: bw6o2zl1
Snippet: We have developed a rapid, accurate, and cost-effective serologic test for SARS-CoV-2 virus, which caused the COVID-19 pandemic, on the basis of antibody-dependent agglutination of antigen-coated latex particles. When validated using plasma samples that are positive or negative for SARS-CoV-2, the agglutination assay detected antibodies against the receptor-binding domain of the spike (S-RBD) or the nucleocapsid protein of SARS-CoV-2 with 100% specificity and ∼98% sensitivity. Furthermore, we
Document: We have developed a rapid, accurate, and cost-effective serologic test for SARS-CoV-2 virus, which caused the COVID-19 pandemic, on the basis of antibody-dependent agglutination of antigen-coated latex particles. When validated using plasma samples that are positive or negative for SARS-CoV-2, the agglutination assay detected antibodies against the receptor-binding domain of the spike (S-RBD) or the nucleocapsid protein of SARS-CoV-2 with 100% specificity and ∼98% sensitivity. Furthermore, we found that the strength of the S-RBD antibody response measured by the agglutination assay correlated with the efficiency of the plasma in blocking RBD binding to the angiotensin-converting enzyme 2 in a surrogate neutralization assay, suggesting that the agglutination assay might be used to identify individuals with virus-neutralizing antibodies. Intriguingly, we found that >92% of patients had detectable antibodies on the day of a positive viral RNA test, suggesting that the agglutination antibody test might complement RNA testing for the diagnosis of SARS-CoV-2 infection.
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