Author: Andres S. Espindola; William Schneider; Kitty F. Cardwell; Yisel Carrillo; Peter R. Hoyt; Stephen M. Marek; Hassan Melouk; Carla D. Garzon
Title: Inferring the presence of aflatoxin-producing Aspergillus flavus strains using RNA sequencing and electronic probes as a transcriptomic screening tool Document date: 2018_7_9
ID: hdwn2fkj_8
Snippet: The genomes from A. flavus AF70 (Accession: JZDT00000000.1) and NRRL3357 167 (Accession: AAIH00000000.2 ) [38] were obtained from Genbank. Sequences for the 168 aflatoxin gene cluster of AF70 (AY510453) and AF36 (AY510455) were also retrieved 169 from GenBank [39] . E-probes 80 nt long were generated using the e-probe pipeline for The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.11.....
Document: The genomes from A. flavus AF70 (Accession: JZDT00000000.1) and NRRL3357 167 (Accession: AAIH00000000.2 ) [38] were obtained from Genbank. Sequences for the 168 aflatoxin gene cluster of AF70 (AY510453) and AF36 (AY510455) were also retrieved 169 from GenBank [39] . E-probes 80 nt long were generated using the e-probe pipeline for The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/365254 doi: bioRxiv preprint from 20 to 24,9 million reads per sequencing run ( Table 1) . The sequenced reads then 202 were mapped to the A. flavus AF70 strain genome to retrieve information about 203 potential up-regulation and down-regulation of genes by using STAR [36] and DESeq2. 204
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