Author: Asif Shajahan; Nitin T. Supekar; Anne S. Gleinich; Parastoo Azadi
Title: Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 Document date: 2020_4_3
ID: f0xsisdg_16
Snippet: We observed both high mannose and complex-type glycans across the N-glycosylation sites but found no hybrid type N-glycans. We quantified the relative intensities of glycans at each site by comparing the area under the curve of each glycopeptide peak on the LC-MS chromatogram. A recent preprint investigated the N-glycosylation on SARS-CoV-2 and reported prevalence of hybrid-type glycans (Watanabe, Y., Allen, J.D., et al. 2020) . In contrast, we o.....
Document: We observed both high mannose and complex-type glycans across the N-glycosylation sites but found no hybrid type N-glycans. We quantified the relative intensities of glycans at each site by comparing the area under the curve of each glycopeptide peak on the LC-MS chromatogram. A recent preprint investigated the N-glycosylation on SARS-CoV-2 and reported prevalence of hybrid-type glycans (Watanabe, Y., Allen, J.D., et al. 2020) . In contrast, we observed a combination of high mannose and complex-type, but no hybrid-type glycans on most of the sites.
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