Author: Kim, Youngeun; Yaseen, Adam B.; Kishi, Jocelyn Y.; Hong, Fan; Saka, Sinem K.; Sheng, Kuanwei; Gopalkrishnan, Nikhil; Schaus, Thomas E.; Yin, Peng
Title: Single-strand RPA for rapid and sensitive detection of SARS-CoV-2 RNA Cord-id: k9f3kw22 Document date: 2020_8_21
ID: k9f3kw22
Snippet: We report the single-strand Recombinase Polymerase Amplification (ssRPA) method, which merges the fast, isothermal amplification of RPA with subsequent rapid conversion of the double-strand DNA amplicon to single strands, and hence enables facile hybridization-based, high-specificity readout. We demonstrate the utility of ssRPA for sensitive and rapid (4 copies per 50 μL reaction within 10 min, or 8 copies within 8 min) visual detection of SARS-CoV-2 RNA spiked samples, as well as clinical sali
Document: We report the single-strand Recombinase Polymerase Amplification (ssRPA) method, which merges the fast, isothermal amplification of RPA with subsequent rapid conversion of the double-strand DNA amplicon to single strands, and hence enables facile hybridization-based, high-specificity readout. We demonstrate the utility of ssRPA for sensitive and rapid (4 copies per 50 μL reaction within 10 min, or 8 copies within 8 min) visual detection of SARS-CoV-2 RNA spiked samples, as well as clinical saliva and nasopharyngeal swabs in VTM or water, on lateral flow devices. The ssRPA method promises rapid, sensitive, and accessible RNA detection to facilitate mass testing in the COVID-19 pandemic.
Search related documents:
Co phrase search for related documents, hyperlinks ordered by date