Author: Pei-Hui Wang; Yun Cheng
Title: Increasing Host Cellular Receptor—Angiotensin-Converting Enzyme 2 (ACE2) Expression by Coronavirus may Facilitate 2019-nCoV Infection Document date: 2020_2_27
ID: epy7774j_15
Snippet: VSV-GFP infection was described in our previous publications (Wang et al., 2018) . Briefly, target cells were washed twice with 37°C DMEM medium, then virus that diluted in serum-free medium were added to the cells. Virus-medium complexes were incubated with cells in normal culture condition. 1-2 hrs later, virus-medium complexes were discarded and replaced with culture medium containing 10% FBS......
Document: VSV-GFP infection was described in our previous publications (Wang et al., 2018) . Briefly, target cells were washed twice with 37°C DMEM medium, then virus that diluted in serum-free medium were added to the cells. Virus-medium complexes were incubated with cells in normal culture condition. 1-2 hrs later, virus-medium complexes were discarded and replaced with culture medium containing 10% FBS.
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