Increased Neurite Orientation-Dispersion and Density in the TgCRND8 Mouse Model of Amyloidosis: Inverse Relation with Functional Connectome Clustering and Modulation by Interleukin-6

Luis M. Colon-Perez; Kristen R. Ibanez; Mallory Suarez; Kristin Torroella; Kelly Acuna; Edward Ofori; Yona Levites; David E. Vaillancourt; Todd E. Golde; Paramita Chakrabarty; Marcelo Febo

We carried out functional MRI scanning at 11.1 Tesla to investigate if in addition to microstructural changes, IL6 or Aβ expression may affect intrinsic brain activity. For seed-based analysis, we chose cortical and hippocampal areas serving spatial and multi-sensory memory functions (Supplemental Figure 2) . Statistical analysis using AFNI 3dMVM did not reveal any significant differences between the groups using this a priori seed-selection approach (data not shown). To supplement the seed-based functional connectivity approach, we conducted model free probabilistic ICA. ICA produced 30 components of which 19 were deemed qualitatively consistent with published resting state functional MRI networks in mice (Bukhari et al., 2017; Sforazzini et al., 2014; Shah et al., 2016) . Figure 7A shows 9 components that included cerebellar, visual, retrosplenial, somatosensory, prefrontal, striatal, midbrain, hippocampal and thalamic regions. Figure 7B shows a comparison of component-24 for a network connecting thalamus, All rights reserved. No reuse allowed without permission.