Author: Rashmi Mohanty; Xinquan Liu; Jae You Kim; Xiujuan Peng; Sahil Bhandari; Jasmim Leal; Dhivya Arasappan; Dennis C. Wylie; Tony Dong; Debadyuti Ghosh
Title: Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment Document date: 2019_6_4
ID: e2uzk4u1_105
Snippet: Nanoparticle diffusion in pancreatic tumor slices using multiple particle tracking Diffusion of the surface functionalized nanoparticles in BxPC3 tumor tissue slices was measured by multiple particle tracking following method described in Dancy et al. 39 . Briefly, the tumor tissue slices were placed on custom microscope slide chambers, and 0.5 µL of 50 µg/mL of yellow-green fluorescent nanoparticles were injected to the ex vivo tumor tissue us.....
Document: Nanoparticle diffusion in pancreatic tumor slices using multiple particle tracking Diffusion of the surface functionalized nanoparticles in BxPC3 tumor tissue slices was measured by multiple particle tracking following method described in Dancy et al. 39 . Briefly, the tumor tissue slices were placed on custom microscope slide chambers, and 0.5 µL of 50 µg/mL of yellow-green fluorescent nanoparticles were injected to the ex vivo tumor tissue using a Hamilton syringe. The slide chambers were sealed using a coverslip, and the particles were allowed to equilibrate with the tumor tissue at 37°C for 15 minutes. The fluorescence intensity of the individual particles was then tracked at a frame rate of 15 frames/sec for 20 sec using an inverted fluorescence IX-83 Olympus microscope with 100X/1.3 NA oil-immersion objective with 65 All rights reserved. No reuse allowed without permission.
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