Identification of peptide coatings that enhance diffusive transport of nanoparticles through the tumor microenvironment

Rashmi Mohanty; Xinquan Liu; Jae You Kim; Xiujuan Peng; Sahil Bhandari; Jasmim Leal; Dhivya Arasappan; Dennis C. Wylie; Tony Dong; Debadyuti Ghosh

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/659524 doi: bioRxiv preprint the original and charge modified clones through tumor-like ECM was measured, and their diffusivity was compared to the theoretical diffusivity of WT in PBS. The original neutral clone exhibits approximately 1.5-fold higher effective diffusivity compared to the positive and negative modifications (Figure 4a) . As a result of the alanine mutations, the hydrophobicity of the sequences has also been changed due to the removal of the polar acidic and basic amino acid residues. A similar decrease in hydrophilicity was also observed during library screening, where highly positive and negative charged peptides (with polar basic and acidic residues) were filtered out with increased rounds (Figures S1a and S1b).