Author: Chin-Yi Chu; Xing Qiu; Matthew N. McCall; Lu Wang; Anthony Corbett; Jeanne Holden-Wiltse; Christopher Slaunwhite; Qian Wang; Christopher Anderson; Alex Grier; Steven R. Gill; Gloria S. Pryhuber; Ann R. Falsey; David J. Topham; Mary T. Caserta; Edward E. Walsh; Thomas J Mariani
Title: Insufficiency in airway interferon activation defines clinical severity to infant RSV infection Document date: 2019_5_20
ID: bx49tbui_24
Snippet: Sequences were aligned by STAR against human genome version of GRCh38 and normalized to FPKM. We used the following non-specific filtering strategy to remove genes with low expression values indistinguishable from background signal. First, we compute , the 95% sample quantile of the expression level of the th gene, for all genes. Next, we defined trimmed maximum log-expressions as = log 2 ( + 1), and removed those genes with < 0 (or equivalently,.....
Document: Sequences were aligned by STAR against human genome version of GRCh38 and normalized to FPKM. We used the following non-specific filtering strategy to remove genes with low expression values indistinguishable from background signal. First, we compute , the 95% sample quantile of the expression level of the th gene, for all genes. Next, we defined trimmed maximum log-expressions as = log 2 ( + 1), and removed those genes with < 0 (or equivalently, < 7.0). After this filtering step, a total of 13,819 genes were selected for further analyses. After removing fifteen samples with total number of mapped reads less than 5 million and ten samples with low average correlation with other samples, 175 samples were selected for further analyses.
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