Influence of different glycoproteins and of the virion core on SERINC5 antiviral activity

William E. Diehl; Mehmet H. Guney; Pyae Phyo Kyawe; Judith M. White; Massimo Pizzato; Jeremy Luban

A previous report indicated that MLV virions pseudotyped with RD114 Env are susceptible to the antiviral effects of SERINC5 (24). However, in the presence of SERINC5 we only observed a modest ~4.5-fold reduction in viral titer of RD114 pseudotyped HIV-1 virions ( Fig. 1A) . In response to this discrepancy, we sought to determine if the viral core modulates susceptibility to SERINC5 antiviral activity. Thus, we tested the same panel of glycoproteins for SERINC5 sensitivity when pseudotyped on different virion cores. First, we tested the SERINC5 sensitivity of the same panel of glycoproteins as in Fig. 1A on MLV viral cores ( Figure 3A and Table 1 ). We observed that the glycoproteins sensitive to SERINC5 restriction on HIV-1 cores (HIV-1, A-MLV, Flu, and Rabies) were also restricted when pseudotyped on MLV cores.