Author: Renata C Fleith; Harriet V Mears; Edward Emmott; Stephen C Graham; Daniel S Mansur; Trevor R Sweeney
Title: IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA Document date: 2018_2_8
ID: j97gul0w_11
Snippet: IFIT complex assembly All complexes were assembled in SEC buffer. IFIT1:IFIT2 and IFIT1:IFIT3-1 mg/mL of each protein was mixed and incubated for one hour at 4 or 30 ï‚°C. IFIT2:IFIT3-0.18 mg/mL of each protein was mixed and incubated for one hour at 37 ï‚°C and concentrated to 2 mg/mL. IFIT1:IFIT2:IFIT3 trimer-0.4 mg/mL of purified IFIT2:IFIT3 complex and 0.2 mg/mL of IFIT1 were incubated for 1 hour at 30 ï‚°C. The complex was concentrated to 3 .....
Document: IFIT complex assembly All complexes were assembled in SEC buffer. IFIT1:IFIT2 and IFIT1:IFIT3-1 mg/mL of each protein was mixed and incubated for one hour at 4 or 30 ï‚°C. IFIT2:IFIT3-0.18 mg/mL of each protein was mixed and incubated for one hour at 37 ï‚°C and concentrated to 2 mg/mL. IFIT1:IFIT2:IFIT3 trimer-0.4 mg/mL of purified IFIT2:IFIT3 complex and 0.2 mg/mL of IFIT1 were incubated for 1 hour at 30 ï‚°C. The complex was concentrated to 3 mg/mL. IFIT1:IFIT2:IFIT3 tetramer-0.4 mg/mL of purified IFIT2:IFIT3 complex and 0.4 mg/mL of IFIT1 were incubated for 1 hour at 30 ï‚°C. The complex was concentrated to 4 mg/mL. Complexes were concentrated using Amicon Ultra 0.5 mL 10 kDa molecular weight cut off filters (Millipore).
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