Selected article for: "Amino acid and NCBI database"
Author: Asiedu Ebenezer
Title: Designing Effective small interfering RNA for Post-Transcriptional Silencing of Human GREM1: A Comprehensive Bioinformatics Approach
  • Document date: 2020_1_24
    • ID: j99qg2a0_11
    Snippet: Human gremlin-1 primary transcript (NM_013372.7) and protein sequence (ID: O60565) were retrieved from the NCBI nucleotide database (https://www.ncbi.nlm.nih.gov/nuccore) and UniProtKB (https://www.uniprot.org/uniprot/) respectively. The mature mRNA that codes for the 184-amino acid gremlin-1 was analyzed using the ExPASy translate tool (https://web.expasy.org/translate/). Fasta file of the human gremlin-1 primary transcript (NM_013372.7) was the.....
    Document: Human gremlin-1 primary transcript (NM_013372.7) and protein sequence (ID: O60565) were retrieved from the NCBI nucleotide database (https://www.ncbi.nlm.nih.gov/nuccore) and UniProtKB (https://www.uniprot.org/uniprot/) respectively. The mature mRNA that codes for the 184-amino acid gremlin-1 was analyzed using the ExPASy translate tool (https://web.expasy.org/translate/). Fasta file of the human gremlin-1 primary transcript (NM_013372.7) was the input data. Reading on both forward and reverse strands were allowed and the output format set to include nucleotide sequences. The precise nucleotide sequences (open reading frame) responsible for coding human gremlin-1 was used as the target for subsequent siRNA design.

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