Comparative Study of Gene Expression Profiling Unravels Functions associated with Pathogenesis of Dengue Infection.

Warsi, Mohiuddin Khan; Kamal, Mohammad Azhar; Baeshen, Mohammed Nabih; Izhari, Mohammad Asrar; Firoz, Ahmad; Mobashir, Mohammad

BACKGROUND Dengue virus is a potential source of propagating dengue hemorrhagic fever. This virus leads to dengue hemorrhagic fever/dengue shock syndrome, benign syndrome, and severe syndrome and due to its infection there occurs alterations at multiple levels such as gene expression and the pathway levels. So, it is critical to understand the pathogenesis of dengue infection in terms of gene expression and the associated functions. METHODS For this purpose, here, we have analyzed the temporal gene expression profiling for dengue hemorrhagic fever dataset at 12, 24, and 48 hours. RESULTS The outcome appears that the dengue hemorrhagic fever evolve differently at different time periods or stages. Counclusions: The change in gene expression pattern increases exponentially from 12 hours to 48 hours and the number of altered functions (pathways) also increases. Wnt, apoptosis, transcription signaling are among the critical pathways which are dominantly altered. In the initial phase (first 12 hours) only two pathways are altered due to dengue infection while in the next 12 hours, eight pathways are altered, and finally in the next 24 hours 11 pathways are altered and most of these 11 pathways are very critical in terms of biological pathways and functions.