Author: Michael T. Parker; Smita Gopinath; Corey E. Perez; Melissa M. Linehan; Jason M. Crawford; Akiko Iwasaki; Brett D. Lindenbach
Title: Innate Immune Priming by cGAS as a Preparatory Countermeasure Against RNA Virus Infection Document date: 2018_10_3
ID: j1gkl43g_13
Snippet: to cytomegalovirus infection was cell type-dependent, despite active cGAS-STING 326 expression. Where cGAMP was detected, levels were on the order of 5 fmol/10 4 327 cells, or ~3x10 5 molecules/cell, which was slightly above their assay's limit of 328 detection (53). As our biochemical and biological assays were both less sensitive 329 than that of Paijo et al., we surmise that the synthesis of cGAMP in response to RNA 330 virus infection is belo.....
Document: to cytomegalovirus infection was cell type-dependent, despite active cGAS-STING 326 expression. Where cGAMP was detected, levels were on the order of 5 fmol/10 4 327 cells, or ~3x10 5 molecules/cell, which was slightly above their assay's limit of 328 detection (53). As our biochemical and biological assays were both less sensitive 329 than that of Paijo et al., we surmise that the synthesis of cGAMP in response to RNA 330 virus infection is below the limit of detection and/or may be rapidly turned over. 331 Alternatively, continuous low-level production of cGAMP in response to endogenous 332 DNA ligands may be more relevant to RNA virus restriction. Clearly, cGAMP assays 333 with improved sensitivity are needed to discern between these possibilities. 334
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