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Author: Christoph Muus; Malte D Luecken; Gokcen Eraslan; Avinash Waghray; Graham Heimberg; Lisa Sikkema; Yoshihiko Kobayashi; Eeshit Dhaval Vaishnav; Ayshwarya Subramanian; Christopher Smillie; Karthik Jagadeesh; Elizabeth Thu Duong; Evgenij Fiskin; Elena Torlai Triglia; Christophe Becavin; Meshal Ansari; Peiwen Cai; Brian Lin; Justin Buchanan; Sijia Chen; Jian Shu; Adam L Haber; Hattie Chung; Daniel T Montoro; Taylor Adams; Hananeh Aliee; Samuel J Allon; Zaneta Andrusivova; Ilias Angelidis; Orr Ashenberg; Kevin Bassler; Christophe Becavin; Inbal Benhar; Joseph Bergenstrahle; Ludvig Bergenstrahle; Liam Bolt; Emelie Braun; Linh T Bui; Mark Chaffin; Evgeny Chichelnitskiy; Joshua Chiou; Thomas M Conlon; Michael S Cuoco; Marie Deprez; David S Fischer; Astrid Gillich; Joshua Gould; Minzhe Guo; Austin J Gutierrez; Arun C Habermann; Tyler Harvey; Peng He; Xiaomeng Hou; Lijuan Hu; Alok Jaiswal; Peiyong Jiang; Theodoros Kapellos; Christin S Kuo; Ludvig Larsson; Michael A Leney-Greene; Kyungtae Lim; Monika Litvinukova; Ji Lu; Leif S Ludwig; Wendy Luo; Henrike Maatz; Elo Maddissoon; Lira Mamanova; Kasidet Manakongtreecheep; Charles-Hugo Marquette; Ian Mbano; Alexi M McAdams; Ross J Metzger; Ahmad N Nabhan; Sarah K Nyquist; Jose Ordovas-Montanes; Lolita Penland; Olivier B Poirion; Segio Poli; CanCan Qi; Daniel Reichart; Ivan Rosas; Jonas Schupp; Rahul Sinha; Rene V Sit; Kamil Slowikowski; Michal Slyper; Neal Smith; Alex Sountoulidis; Maximilian Strunz; Dawei Sun; Carlos Talavera-Lopez; Peng Tan; Jessica Tantivit; Kyle J Travaglini; Nathan R Tucker; Katherine Vernon; Marc H Wadsworth; Julia Waldman; Xiuting Wang; Wenjun Yan; Ali Onder Yildirim; William Zhao; Carly G K Ziegler; Aviv Regev
Title: Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells
  • Document date: 2020_4_20
    • ID: nkql7h9x_81
    Snippet: Immunohistochemistry analysis was performed on 4% PFA fixed, OCT embedded tissue sections from human explant donors. Briefly, cells were permeabilized with 5% Triton-X in PBS. Slides were either not treated with antigen retrieval or antigen retrieval was performed with the Tris-Citrate buffer as needed (Supplementary Table 4) . Slides were incubated overnight with primary antibodies at indicated concentrations (Supplementary Table 4 ) in donkey s.....
    Document: Immunohistochemistry analysis was performed on 4% PFA fixed, OCT embedded tissue sections from human explant donors. Briefly, cells were permeabilized with 5% Triton-X in PBS. Slides were either not treated with antigen retrieval or antigen retrieval was performed with the Tris-Citrate buffer as needed (Supplementary Table 4) . Slides were incubated overnight with primary antibodies at indicated concentrations (Supplementary Table 4 ) in donkey serum with 5% Triton-X in PBS. Slides were treated with Alexa-Fluor secondary antibodies mixed with DAPI at 1:500 concentration in donkey serum with 5% Triton-X in PBS for 1 hour at room temperature. Slides were mounted and imaged on a confocal microscope.

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