Selected article for: "biological significance and direct binding"
Author: Ge, Mengyu; Molt, Robert W.; Jenkins, Huw T.; Blackburn, G. Michael; Jin, Yi; Antson, Alfred A.
Title: Octahedral Trifluoromagnesate, an Anomalous Metal Fluoride Species, Stabilizes the Transition State in a Biological Motor
  • Cord-id: ex938es2
  • Document date: 2021_2_17
    • ID: ex938es2
    Snippet: [Image: see text] Isoelectronic metal fluoride transition state analogue (TSA) complexes, MgF(3)(–) and AlF(4)(–), have proven to be immensely useful in understanding mechanisms of biological motors utilizing phosphoryl transfer. Here we report a previously unobserved octahedral TSA complex, MgF(3)(H(2)O)(−), in a 1.5 Å resolution Zika virus NS3 helicase crystal structure. (19)F NMR provided independent validation and also the direct observation of conformational tightening resulting from
    Document: [Image: see text] Isoelectronic metal fluoride transition state analogue (TSA) complexes, MgF(3)(–) and AlF(4)(–), have proven to be immensely useful in understanding mechanisms of biological motors utilizing phosphoryl transfer. Here we report a previously unobserved octahedral TSA complex, MgF(3)(H(2)O)(−), in a 1.5 Å resolution Zika virus NS3 helicase crystal structure. (19)F NMR provided independent validation and also the direct observation of conformational tightening resulting from ssRNA binding in solution. The TSA stabilizes the two conformations of motif V of the helicase that link ATP hydrolysis with mechanical work. DFT analysis further validated the MgF(3)(H(2)O)(−) species, indicating the significance of this TSA for studies of biological motors.

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