Author: Armin Ensser; Klaus Ueberla
Title: Determination of daily reproduction numbers of SARS-CoV2 based on death cases suggests more rapid initial spread in Italy and the United States Document date: 2020_3_31
ID: 4izymiy4_15
Snippet: Standardization of the assay. The initial nonkinetic Alu-gag preamplification, with substrates and enzyme in excess, produced heterogeneous amplicons of varying length ( Fig. 2A) . Aliquots of these amplicons, or matched aliquots of nonpreamplified standards and unknowns, were then subjected to a quantitative kinetic PCR that detects RU5 DNA within the HIV-1 LTR and produces homogeneous amplicons 100 bp in length (Fig. 2B) ......
Document: Standardization of the assay. The initial nonkinetic Alu-gag preamplification, with substrates and enzyme in excess, produced heterogeneous amplicons of varying length ( Fig. 2A) . Aliquots of these amplicons, or matched aliquots of nonpreamplified standards and unknowns, were then subjected to a quantitative kinetic PCR that detects RU5 DNA within the HIV-1 LTR and produces homogeneous amplicons 100 bp in length (Fig. 2B) .
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