Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Kenneth N. Hass; Mengdi Bao; Qian He; Myeongkee Park; Peiwu Qin; Ke Du

We have shown that the PDMS micropillars provide a significant increase in solid-state capture probe binding than the flat surface. The increased ssDNA capture probe capacity can extend the detection limit and also dynamic range. 23, 24 In addition, the micropillar array increases the interaction between biomolecules and the substrate, as the surface area in contact with any given solution is significantly higher. To further increase the probe binding capacity, we can increase the aspect ratio of PDMS micropillars and the microchannel length. Indeed, the channel was Figure 4 : Released ssDNA reporter probe integrated intensity for flat and micropillar channels with a DNA input load of 1×10 4 , 1×10 5 , and 1×10 6 nmoles, respectively. UV photocleavage was used to retrieve the ssDNA reporter probes. Error bars represent 5%-95% confidence intervals and middle line is mean.