Author: Jun Wu; Dingyun Song; Zhongwen Li; Baojie Guo; Yani Xiao; Wenjing Liu; Lingmin Liang; Chunjing Feng; Tingting Gao; Zai Wang; Jianyan Wen; Shengnan Yang; Peipei Liu; Lei Wang; Yukai Wang; Liang Peng; Guihai Feng; Shiqiang Huang; Glyn Nigel Stacey; Wei Li; Yan Huo; Ronghua Jin; Qi Zhou; Liu Wang; Baoyang Hu; Huaping Dai; Jie Hao
Title: Immunity-and-Matrix-Regulatory Cells Derived from Human Embryonic Stem Cells Safely and Effectively Treat Mouse Lung Injury and Fibrosis Document date: 2020_4_16
ID: 8dpla6jt_5
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.15.042119 doi: bioRxiv preprint Cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X-100 for 15 241 min and blocked with 2% BSA for 60 min at room temperature. Then, the cells were stained with 1: USA; IPVH00010). The membranes were blocked at room temperature with 1% BSA for 1 h and 258 incubated overnig.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.15.042119 doi: bioRxiv preprint Cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X-100 for 15 241 min and blocked with 2% BSA for 60 min at room temperature. Then, the cells were stained with 1: USA; IPVH00010). The membranes were blocked at room temperature with 1% BSA for 1 h and 258 incubated overnight at 4°C with primary antibodies: rabbit Collagen I antibody (Cell Signaling 259 Technology, 84336), mouse E-cadherin antibody (Abcam, ab1416) and mouse β-actin antibody (Sigma-260
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