Selected article for: "LAMP amplification and RNA template"
Author: Gun-Soo Park; Keunbon Ku; Seung-Hwa Beak; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Jin-Soo Maeng
Title: Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
  • Document date: 2020_3_12
    • ID: 1qn5y4gc_32
    Snippet: showed good sensitivity to cDNA template (Supplementary Figure 1) unlike to RNA. Observed difference of sensitivity to RNA and cDNA seems significant even we account slight amplification that . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.09.983064 doi: bioRxiv preprint might happened during reverse tr.....
    Document: showed good sensitivity to cDNA template (Supplementary Figure 1) unlike to RNA. Observed difference of sensitivity to RNA and cDNA seems significant even we account slight amplification that . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.09.983064 doi: bioRxiv preprint might happened during reverse transcription. The reason may include stochastic nature of forming proper LAMP amplification intermediate -"dumbbell" structure [24] , higher stability of DNA:RNA double strand than DNA:DNA double strand and more.

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