Author: Willcocks, Margaret M.; Carter, Michael J.
Title: Identification and sequence determination of the capsid protein gene of human astrovirus serotype 1 Cord-id: f2jf7byg Document date: 2006_1_17
ID: f2jf7byg
Snippet: Abstract We present the sequence of an open reading frame (ORF) at the 3′ end of human astrovirus serotype 1. Primer extension experiments showed that the RNA expressing this gene is shorter than the complete ORF, and could form a protein of M (r) 85 540. The protein was expressed by recombinant baculovirus and was recognized by antiâ€virion serum, indicating a structural role. Sequence comparison indicates that astrovirus serotypes 1 and 2 differ markedly in the Câ€terminal half of the prot
Document: Abstract We present the sequence of an open reading frame (ORF) at the 3′ end of human astrovirus serotype 1. Primer extension experiments showed that the RNA expressing this gene is shorter than the complete ORF, and could form a protein of M (r) 85 540. The protein was expressed by recombinant baculovirus and was recognized by antiâ€virion serum, indicating a structural role. Sequence comparison indicates that astrovirus serotypes 1 and 2 differ markedly in the Câ€terminal half of the protein but are well conserved towards the Nâ€terminus.
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