Author: David E. Ochayon; Ayad Ali; Pablo C. Alarcon; Durga Krishnamurthy; Leah C. Kottyan; Michael Borchers; Stephen N. Waggoner
Title: Interleukin-33 promotes type 1 cytokine expression via p38 MAPK in human natural killer cells Document date: 2019_9_20
ID: iuhhuy53_28
Snippet: In vitro culture of NK cells. Enriched NK cells were cultured in SCGM media (Cellgenix®, Freiburg, Germany) supplemented with IL-2 (400 U/ml, Peprotech, Rocky Hill, NJ), 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 U/ml streptomycin, 1% nonessential amino acids, 1% sodium pyruvate, 2mM L-Glutamine and 10 mM HEPES. In experiments, NK cells were either used directly after blood isolation.....
Document: In vitro culture of NK cells. Enriched NK cells were cultured in SCGM media (Cellgenix®, Freiburg, Germany) supplemented with IL-2 (400 U/ml, Peprotech, Rocky Hill, NJ), 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 U/ml streptomycin, 1% nonessential amino acids, 1% sodium pyruvate, 2mM L-Glutamine and 10 mM HEPES. In experiments, NK cells were either used directly after blood isolation or after 7 to 30 days of ex vivo culture. Each scenario produced similar results.
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