Author: David E. Ochayon; Ayad Ali; Pablo C. Alarcon; Durga Krishnamurthy; Leah C. Kottyan; Michael Borchers; Stephen N. Waggoner
Title: Interleukin-33 promotes type 1 cytokine expression via p38 MAPK in human natural killer cells Document date: 2019_9_20
ID: iuhhuy53_7
Snippet: Antibody blockade of the IL-33 receptor, ST2, abrogated the enhancing effect of IL-33 on IL-12-induced IFN-ï§ expression, consistent with previous studies 16 , but had no measurable effect on IFN-ï§ induced by IL-12 alone (Fig. Figure 2 . IL-33 enhances IL-12-induced cytokine expression in primary human NK cells. Enriched primary human NK cells were stimulated with combinations of IL-12 and IL-33 (doses listed in ng/mL) for 16 hours prior to .....
Document: Antibody blockade of the IL-33 receptor, ST2, abrogated the enhancing effect of IL-33 on IL-12-induced IFN-ï§ expression, consistent with previous studies 16 , but had no measurable effect on IFN-ï§ induced by IL-12 alone (Fig. Figure 2 . IL-33 enhances IL-12-induced cytokine expression in primary human NK cells. Enriched primary human NK cells were stimulated with combinations of IL-12 and IL-33 (doses listed in ng/mL) for 16 hours prior to ELISA determination of (A) IFN-ï§, (B) TNF or GM-CSF expression in cell-free supernatant. Results from one representative iteration of (A) ten or (B) five similar experiments using cells from different subjects is shown (each dot is biological replicate and columns represent mean). Results of ordinary one-way ANOVA (B) assessment of group means with multiple comparisons (A) is shown. Two-way ANOVA was used to assess synergistic cytokine interactions (reported in the Results text).
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