Selected article for: "cell binding and enhanced cell binding"

Author: Zhang, Li; Cao, Lei; Gao, Xingsu; Zheng, Binyang; Deng, Yongqiang; Li, Jing-Xin; Feng, Rui; Bian, Qian; Guo, Xi-Ling; Wang, Nan; Qiu, Hong-Ying; Wang, Lei; Cui, Zhen; Ye, Qing; Chen, Geng; Lu, Kui-Kui; Chen, Yin; Chen, Yu-Tao; Pan, Hong-Xing; Zhu, Bao-Li; Qin, Cheng-Feng; Wang, Xiangxi; Zhu, Feng-Cai
Title: A proof of concept for neutralizing antibody-guided vaccine design against SARS-CoV-2
  • Cord-id: pztcrun3
  • Document date: 2020_9_23
  • ID: pztcrun3
    Snippet: Mutations and transient conformational movements of receptor binding domain (RBD) that make neutralizing epitopes momentarily unavailable, present immune escape routes to SARS-CoV-2. To mitigate viral escape, we developed a cocktail of neutralizing antibodies (NAbs) targeting epitopes located on different domains of spike (S) protein. Screening of a library of monoclonal antibodies generated from peripheral blood mononuclear cells of COVID-19 convalescent patients yielded potent NAbs, targeting
    Document: Mutations and transient conformational movements of receptor binding domain (RBD) that make neutralizing epitopes momentarily unavailable, present immune escape routes to SARS-CoV-2. To mitigate viral escape, we developed a cocktail of neutralizing antibodies (NAbs) targeting epitopes located on different domains of spike (S) protein. Screening of a library of monoclonal antibodies generated from peripheral blood mononuclear cells of COVID-19 convalescent patients yielded potent NAbs, targeting N-terminal domain (NTD) and RBD domain of S, effective at nM concentrations. Remarkably, combination of RBD-targeting NAbs and NTD-binding NAb, FC05, dramatically enhanced the neutralization potency in cell-based assays and animal model. Results of competitive SPR assays and cryo-EM structures of Fabs bound to S unveil determinants of immunogenicity. Combinations of immunogens, identified in NTD and RBD of S, when immunized in rabbits elicited potent protective immune responses against SARS-CoV-2. These results provide a proof-of-concept for neutralization-based immunogen design targeting SARS-CoV-2 NTD and RBD. One sentence summaryImmunogens identified in the NTD and RBD of the SARS-CoV-2 spike protein using a cocktail of non-competing NAbs when injected in rabbits elicited a potent protective immune response against SARS-CoV-2.

    Search related documents:
    Co phrase search for related documents
    • Try single phrases listed below for: 1
    Co phrase search for related documents, hyperlinks ordered by date