Author: Kenneth N. Hass; Mengdi Bao; Qian He; Myeongkee Park; Peiwu Qin; Ke Du
Title: Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing Document date: 2020_3_20
ID: d840uu3e_25
Snippet: Fluorescence Quantification: To quantify the fluorescence intensity of the ssDNA reporter probes, a custom designed fluorometer was used and has been reported before. 21,50 Briefly, a continuous wave laser with an emission peak at 488 nm (Sapphire 488 LP, Coherent) was aligned under a reservoir filled with fluorescent molecules. The fluorescence signal was collected by an off-axis parabolic mirror and a fiber coupled mini USB spectrometer (USB 20.....
Document: Fluorescence Quantification: To quantify the fluorescence intensity of the ssDNA reporter probes, a custom designed fluorometer was used and has been reported before. 21,50 Briefly, a continuous wave laser with an emission peak at 488 nm (Sapphire 488 LP, Coherent) was aligned under a reservoir filled with fluorescent molecules. The fluorescence signal was collected by an off-axis parabolic mirror and a fiber coupled mini USB spectrometer (USB 2000+, Ocean Optics). To reduce background noise from the excitation light, a 488 nm notch filter (Thorlabs, Inc.) was placed in front of the optical fiber. author/funder. All rights reserved. No reuse allowed without permission.
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