In-vivo targeted tagging of RNA isolates cell specific transcriptional responses to environmental stimuli and identifies liver-to-adipose RNA transfer

J. Darr; M. Lassi; Archana Tomar; R. Gerlini; F. Scheid; M. Hrabe de Angelis; M. Witting; R. Teperino

In kidney, where proximal renal epithelial cells are labelled, GO annotation and gene set 251 enrichment analysis using Enrichr [45] demonstrated an enrichment for genes localized to the 252 brush boarder membrane, along with a few more general terms found enriched also in input such 253 as mitochondria, focal adhesion and genes specific to or highly expressed in the kidney ( Figure 254 4b-c, Sup. table 3). The brush boarder membrane in the kidney is a unique feature of proximal 255 renal epithelial cells [46] , and among pulled-down transcripts, the Solute Carrier Family 9 member 256 A3 (Slc9a3) is one of its specific markers. Slc9a3 is the sodium-hydrogen antiporter 3, which is 257 highly expressed in the proximal tubule and allows active transport of sodium to the cell. Terms The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/670398 doi: bioRxiv preprint Taken together these results support specific labelling of renal proximal tubule epithelial cells in 280 the kidney and of hepatocytes in the liver, with enrichment of their transcriptomes in pulled-down 281 RNA and specific depletion for genes associated with other cell types found in these organs.