Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously Document date: 2020_2_22
ID: 0vjs2w3l_5
Snippet: Nucleic acid (DNA/RNA) was extracted using a commercial kit (RC311-C1, Vazyme) according to the manufacturer's protocol. Briefly, 500 µL samples preserved in R503 was first added into a 1.5 ml EP tube containing 200 µL of absolute ethanol and 200 µL samples preserved in Hank's solution was added into a 1.5 ml EP tube containing 500 µL lysis buffer......
Document: Nucleic acid (DNA/RNA) was extracted using a commercial kit (RC311-C1, Vazyme) according to the manufacturer's protocol. Briefly, 500 µL samples preserved in R503 was first added into a 1.5 ml EP tube containing 200 µL of absolute ethanol and 200 µL samples preserved in Hank's solution was added into a 1.5 ml EP tube containing 500 µL lysis buffer.
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