Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_14
Snippet: The pseudovirus samples were obtained from Zeesan Biotech (Xiamen, China) and the standard samples were freshly prepared by step-wise dilution of pseudovirus in fetal calf serum purchased from Thermofisher (Massachusetts, USA) before nucleic acid extraction experiments. 200 μL of as-prepared standard samples with a known copy number of viral particles (down to 10 copies) were incubated with 400 μL lysis/binding buffer (1 M NaI, 2.5 M NaCl, 10% .....
Document: The pseudovirus samples were obtained from Zeesan Biotech (Xiamen, China) and the standard samples were freshly prepared by step-wise dilution of pseudovirus in fetal calf serum purchased from Thermofisher (Massachusetts, USA) before nucleic acid extraction experiments. 200 μL of as-prepared standard samples with a known copy number of viral particles (down to 10 copies) were incubated with 400 μL lysis/binding buffer (1 M NaI, 2.5 M NaCl, 10% Triton X-100, 40% polyethylene glycol 8000, 25 mM EDTA) and 40 μg pcMNPs for 10 min at room temperature on a rotating shaker.
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