Selected article for: "confocal microscope and fluorescence microscope"
Author: Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai
Title: DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies
  • Document date: 2019_3_23
    • ID: 5bw7umap_62
    Snippet: Loading efficiencies of different cargos on-surface or in-core were quantified through the fluorescence-based assay of dye-labeled DNA strands (5'Quasar570-compR, 5'Quasar705-compG, 5'Quasar670-compB, Fig. Supplementary 2f) , peptides (C-terminus FITC-labeled peptide, LifeTein, LLC) or proteins (FITC-labeled human IgG, Sigma #SLBW7799), after etching of particles by dimethyl sulfoxide (DMSO) and the dilution with 9x volume of water. The fluoresce.....
    Document: Loading efficiencies of different cargos on-surface or in-core were quantified through the fluorescence-based assay of dye-labeled DNA strands (5'Quasar570-compR, 5'Quasar705-compG, 5'Quasar670-compB, Fig. Supplementary 2f) , peptides (C-terminus FITC-labeled peptide, LifeTein, LLC) or proteins (FITC-labeled human IgG, Sigma #SLBW7799), after etching of particles by dimethyl sulfoxide (DMSO) and the dilution with 9x volume of water. The fluorescence signal was detected by a plate reader (Tecan Spark) and analyzed using a calibration curve with the normalization from OD550. Functionalized microparticles with fluorescent labels were imaged through a spinning disk confocal fluorescence microscope (Nikon All rights reserved. No reuse allowed without permission.

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