Author: Wiley, Luke A; Anfinson, Kristin R; Cranston, Cathryn M; Kaalberg, Emily E; Collins, Malia M; Mullins, Robert F; Stone, Edwin M; Tucker, Budd A
Title: Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy. Cord-id: na7fbchf Document date: 2017_1_1
ID: na7fbchf
Snippet: This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)-derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how, using xeno-free reagents in an ISO class 5 environment, one can isolate and culture dermal fibroblasts, generate iPSCs, and derive autologous retinal cells via 3-D differentiation. The universal methods described herein for the isolation of dermal fibroblasts and g
Document: This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)-derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how, using xeno-free reagents in an ISO class 5 environment, one can isolate and culture dermal fibroblasts, generate iPSCs, and derive autologous retinal cells via 3-D differentiation. The universal methods described herein for the isolation of dermal fibroblasts and generation of iPSCs can be employed regardless of disease, tissue, or cell type of interest. © 2017 by John Wiley & Sons, Inc.
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