Selected article for: "dna control and positive dna control"
Author: Yosuke Hirotsu; Hitoshi Mochizuki; Masao Omata
Title: Double-Quencher Probes Improved the Detection Sensitivity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by One-Step RT-PCR
  • Document date: 2020_3_20
    • ID: 2gokv7id_27
    Snippet: We confirmed that the N1 site of NIID assay was not detected when using less than 10 copies of DNA positive control. In addition, as observed, Ct value was also high by the N1 site of the NIID assay. Similar to these observations, Shirato et al. also demonstrated less sensitivity of the N1 site of NIID assay using RNA positive control. The assessment of N1 site was originally reported by the groups in Germany. To improve the detection sensitivity.....
    Document: We confirmed that the N1 site of NIID assay was not detected when using less than 10 copies of DNA positive control. In addition, as observed, Ct value was also high by the N1 site of the NIID assay. Similar to these observations, Shirato et al. also demonstrated less sensitivity of the N1 site of NIID assay using RNA positive control. The assessment of N1 site was originally reported by the groups in Germany. To improve the detection sensitivity, we recommended the use of a double-quencher probe for detection of SARS-CoV-2, especially in the N1 site.

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