Selected article for: "mature seq and miRNA seq"
Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo
  • Document date: 2018_4_6
    • ID: 1pbshnw9_15
    Snippet: For each miRBase miRNA, we calculated the percentage of trimmed pre-miRNAseq reads that ended between 8-15 nt upstream of the 3' end of the 3p miRNA or downstream of the 5' end of the 5p miRNA. We identified pre-miRNAs with >1% of such cleavage events in the 3p arm as putative ac-pre-miRNAs. For cleavage events in the 5p arm, we took a more conservative approach, requiring 5% of reads to terminate in this region and > 50% of mature miRNA-seq read.....
    Document: For each miRBase miRNA, we calculated the percentage of trimmed pre-miRNAseq reads that ended between 8-15 nt upstream of the 3' end of the 3p miRNA or downstream of the 5' end of the 5p miRNA. We identified pre-miRNAs with >1% of such cleavage events in the 3p arm as putative ac-pre-miRNAs. For cleavage events in the 5p arm, we took a more conservative approach, requiring 5% of reads to terminate in this region and > 50% of mature miRNA-seq reads in the 3p arm compared to 5p arm. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/294488 doi: bioRxiv preprint

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