Selected article for: "FIP inner primer and forward FIP inner primer"
Author: Jonathan L Schmid-Burgk; David Li; David Feldman; Mikolaj Slabicki; Jacob Borrajo; Jonathan Strecker; Brian Cleary; Aviv Regev; Feng Zhang
Title: LAMP-Seq: Population-Scale COVID-19 Diagnostics Using a Compressed Barcode Space
  • Document date: 2020_4_8
    • ID: 68ps3uit_8
    Snippet: RT-LAMP reactions have been demonstrated to be highly sensitive for sequence-specific viral nucleic acid detection (Lamb et al., 2020; Yang et al., 2020; Zhang et al., 2020) , even from unpurified samples (Estrela et al., 2019) . To establish a barcoded RT-LAMP reaction, we inserted barcode sequences into the forward inner primer (FIP), which enables generation of repeatedly barcoded palindromic amplification products (Fig. 1B) . To limit the num.....
    Document: RT-LAMP reactions have been demonstrated to be highly sensitive for sequence-specific viral nucleic acid detection (Lamb et al., 2020; Yang et al., 2020; Zhang et al., 2020) , even from unpurified samples (Estrela et al., 2019) . To establish a barcoded RT-LAMP reaction, we inserted barcode sequences into the forward inner primer (FIP), which enables generation of repeatedly barcoded palindromic amplification products (Fig. 1B) . To limit the number of unique barcode primers needed for testing a large number of samples, a compressed barcode space can be used ( Fig. 1C ) (see below for details) as long as a small fraction of samples is expected to be positive during population scale testing.

    Search related documents:
    Co phrase search for related documents
    • amplification product and large number: 1
    • amplification product and population scale: 1
    • amplification product and population scale testing: 1
    • amplification product and RT LAMP reaction: 1
    • barcode primer and FIP forward inner primer: 1
    • barcode primer and forward inner primer: 1
    • barcode primer and inner primer: 1
    • barcode primer and RT LAMP reaction: 1
    • barcode space and population scale: 1, 2
    • barcode space and population scale testing: 1, 2
    • FIP forward inner primer and forward inner primer: 1, 2
    • FIP forward inner primer and inner primer: 1, 2
    • FIP forward inner primer and RT LAMP reaction: 1
    • forward inner primer and inner primer: 1, 2
    • forward inner primer and RT LAMP reaction: 1
    • inner primer and RT LAMP reaction: 1
    • large number and population scale testing: 1, 2, 3, 4, 5, 6
    • large number and RT LAMP reaction: 1
    • large number and sample large number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22