Author: Jacob Peter Matson; Amy M. House; Gavin D. Grant; Huaitong Wu; Joanna Perez; Jeanette Gowen Cook
Title: Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence Document date: 2019_2_22
ID: dsbucda9_50
Snippet: Cells were plated with siRNA and counted 48 hours or 72 hours later after dissociating with trypsin using a Luna II automated cell counter (Logos Biosystems). Each treatment was done with 3 biological replicates and each dish in the replicate was counted twice as technical replicates. Doubling time was calculated using Prism 8 (GraphPad) regression analysis: exponential growth equation. Doubling times from the 3 biological replicates were average.....
Document: Cells were plated with siRNA and counted 48 hours or 72 hours later after dissociating with trypsin using a Luna II automated cell counter (Logos Biosystems). Each treatment was done with 3 biological replicates and each dish in the replicate was counted twice as technical replicates. Doubling time was calculated using Prism 8 (GraphPad) regression analysis: exponential growth equation. Doubling times from the 3 biological replicates were averaged, and then the average doubling time was multiplied by the cell cycle phase percentages to obtain cell cycle phase hours.
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