Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo Document date: 2020_4_5
ID: aju2nr9x_34
Snippet: Quantification of RBD levels with ELISA 1 × 10 5 293T cells were seeded in each well in a 24-well plate and grew overnight. The cells were treated with 100 ng per well mRNAs encoding the RBD antigen using TT3 nanoparticles as mentioned above. After overnight incubation, cells were lysed in each well. The cell lysis was retrieved and centrifuged at 12000×rpm for 10min. EZview Red ANTI-FLAG M2 Affinity Gel was used to concentrate the FLAG-tagged .....
Document: Quantification of RBD levels with ELISA 1 × 10 5 293T cells were seeded in each well in a 24-well plate and grew overnight. The cells were treated with 100 ng per well mRNAs encoding the RBD antigen using TT3 nanoparticles as mentioned above. After overnight incubation, cells were lysed in each well. The cell lysis was retrieved and centrifuged at 12000×rpm for 10min. EZview Red ANTI-FLAG M2 Affinity Gel was used to concentrate the FLAG-tagged RBD in the pellet following the manufacturer's protocol. RBD was eluted into 0.1M glycine HCl at pH 3.5 and diluted 10 times before coating a 96-well immunoplate (Thermo/Nunc). After blocking, primary rabbit anti-FLAG antibody (ab1162, abcam) at 1:1000 dilution was added, author/funder. All rights reserved. No reuse allowed without permission.
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