Author: Hu, Hui; Tao, Ling; Wang, Yabin; Chen, Liying; Yang, Jihong; Wang, Hanzhong
Title: Enhancing immune responses against SARS-CoV nucleocapsid DNA vaccine by co-inoculating interleukin-2 expressing vector in mice Cord-id: gi7ktelw Document date: 2009_7_5
ID: gi7ktelw
Snippet: The immunogenicity of SARS-CoV nucleocapsid DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2) were investigated. DNA vaccine plasmids, pcDNA-N and pcDNA-IL2, were constructed and inoculated into BALB/c mice with or without pcDNA-IL2 by intramuscular injection. Cellular and humoral immune responses were assessed by indirect ELISA, lymphocyte proliferation assays, ELISPOT and FACS. The nucleocapsid DNA vaccine had good immunogenicity and can induce specific humoral and cellular
Document: The immunogenicity of SARS-CoV nucleocapsid DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2) were investigated. DNA vaccine plasmids, pcDNA-N and pcDNA-IL2, were constructed and inoculated into BALB/c mice with or without pcDNA-IL2 by intramuscular injection. Cellular and humoral immune responses were assessed by indirect ELISA, lymphocyte proliferation assays, ELISPOT and FACS. The nucleocapsid DNA vaccine had good immunogenicity and can induce specific humoral and cellular immunity in BALB/c mice, while IL-2 plays an immunoadjuvant role and enhances specific immune responses. This study provides a frame of reference for the design of DNA vaccines against SARS-CoV.
Search related documents:
Co phrase search for related documents- acute respiratory syndrome and adjuvant cytokine: 1, 2, 3
- acute respiratory syndrome and adjuvant cytokine gene: 1
- acute respiratory syndrome and adjuvant vaccine: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- acute respiratory syndrome and administration co: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- acute respiratory syndrome and long last immunity: 1
- acute respiratory syndrome and low immunogenicity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- acute respiratory syndrome and low number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62
- acute respiratory syndrome and lymphocyte proliferation: 1, 2, 3
- adjuvant vaccine and administration co: 1, 2, 3, 4, 5, 6, 7, 8
- adjuvant vaccine and low immunogenicity: 1, 2, 3, 4
- adjuvant vaccine and lymphocyte proliferation: 1, 2, 3, 4, 5
- adjuvant vaccine and lymphocyte proliferation assay: 1
- administration co and lymphocyte proliferation: 1
Co phrase search for related documents, hyperlinks ordered by date