Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo Document date: 2020_4_5
ID: aju2nr9x_21
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.01.019877 doi: bioRxiv preprint Figure 5 . NASAR mRNAs encoding potential SARS-CoV-2 antigens. a, Relative RBD antigen level in 293T cells quantified by ELISA (n=3). b, Fluorescent microscopy imaging of S and RBD antigens in 293T cells. Scale bar = 100µm. c, Quantification of luciferase expression in vivo after i.m. injection to mice. MC.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.01.019877 doi: bioRxiv preprint Figure 5 . NASAR mRNAs encoding potential SARS-CoV-2 antigens. a, Relative RBD antigen level in 293T cells quantified by ELISA (n=3). b, Fluorescent microscopy imaging of S and RBD antigens in 293T cells. Scale bar = 100µm. c, Quantification of luciferase expression in vivo after i.m. injection to mice. MC3-NASAR luciferase and TT3-NASAR luciferase mRNAs (n=6). d, Confocal microscopy imaging of S and RBD antigens expressed in mouse muscle tissues after i.m. injection. Scale bar = 100µm. All data are presented as the mean ± s.d. Statistical significance in a and c was analyzed by the twotailed Student's t-test. **P < 0.01; ***P < 0.001.
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