Author: Gray, David
Title: Overview of Protein Expression by Mammalian Cells Cord-id: bb0ag8yz Document date: 1997_5_1
ID: bb0ag8yz
Snippet: This unit reviews the stages involved in protein production in mammalian cells using a stableâ€expression approach. Choice of cell type is discussed, as is transfection of the host cells, methods for selection and amplification of transformants, and growth of cells at appropriate scale for protein production. Since postâ€transcriptional modification and intracellular protein transportation are important features of recombinantâ€protein production in mammalian cells, some description of these
Document: This unit reviews the stages involved in protein production in mammalian cells using a stableâ€expression approach. Choice of cell type is discussed, as is transfection of the host cells, methods for selection and amplification of transformants, and growth of cells at appropriate scale for protein production. Since postâ€transcriptional modification and intracellular protein transportation are important features of recombinantâ€protein production in mammalian cells, some description of these mechanisms is included.
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