Author: Leikina, Evgenia; Onaran, H.Ongun; Zimmerberg, Joshua
Title: Acidic pH induces fusion of cells infected with baculovirus to form syncytia Cord-id: gnnh8mt0 Document date: 1992_6_15
ID: gnnh8mt0
Snippet: The enveloped baculovirus⧹insect cell system has been used extensively for expression of recombinant proteins. including viral fusion proteins. We tested wildâ€type baculovirus for endogenous fusion protein activity. Syncytia formation, dye transfer, and capacitance changes were observed after incubating infected Spodoptera frugiperda cells in acidic media, consistent with fusion protein activity. Only a short acidic pulse or 10 s is needed to trigger syncytia formation. Identical results wer
Document: The enveloped baculovirus⧹insect cell system has been used extensively for expression of recombinant proteins. including viral fusion proteins. We tested wildâ€type baculovirus for endogenous fusion protein activity. Syncytia formation, dye transfer, and capacitance changes were observed after incubating infected Spodoptera frugiperda cells in acidic media, consistent with fusion protein activity. Only a short acidic pulse or 10 s is needed to trigger syncytia formation. Identical results were obtained with recombinant baculovirus. This new system convenient for studying pH activated cellâ€cell fusion. However, using this enveloped virus to study the mechanism of recombinant fusion proteins requires caution.
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