Author: Jacob Peter Matson; Amy M. House; Gavin D. Grant; Huaitong Wu; Joanna Perez; Jeanette Gowen Cook
Title: Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence Document date: 2019_2_22
ID: dsbucda9_17
Snippet: The time Cdc6 first appeared in G1 is marked "rise," and the time of maximum nuclear Cdc6 in G1 is marked "peak" (Fig. 6B) . Cells began S phase as measured by the first appearance of PCNA foci about 20-30 minutes after this peak (data not shown). The length of time Cdc6 was present in G1 is the time between nuclear Cdc6 rise and peak, and represents the "licensing window" for MCM loading in G1 (Fig. 6B) . Cells reentering the first cell cycle af.....
Document: The time Cdc6 first appeared in G1 is marked "rise," and the time of maximum nuclear Cdc6 in G1 is marked "peak" (Fig. 6B) . Cells began S phase as measured by the first appearance of PCNA foci about 20-30 minutes after this peak (data not shown). The length of time Cdc6 was present in G1 is the time between nuclear Cdc6 rise and peak, and represents the "licensing window" for MCM loading in G1 (Fig. 6B) . Cells reentering the first cell cycle after G0 had about double the licensing window of cells in the second cell cycle, and about twice as much time in G1 overall (Cdc6 peak time) as cells in the second cell cycle (Fig. 6C, D) .
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